2010

CAST and ELKS proteins: structural and functional determinants of the presynaptic active zone.

Hida, Y.,Ohtsuka, T.
J. Biochem. 2010 148: 131-137.NEW

Reduced expression of the ATRX gene, a chromatin-remodeling factor, causes hippocampal dysfunction in mice.

Nogami, T., Beppu, H., Tokoro, T., Moriguchi, S., Shioda, N., Fukuaga, K., Ohtsuka, T., Ishii, I., Sasahara, M., Shimada, Y., Nishijo, H., Li, E., and Kitajima, I.
Hippocampus 2010 In press. NEW

2009

Involvement of ELKS, an active zone protein, in exocytotic release from RBL-2H3 cells.

Nomura, H., Ohtsuka, T., Tadokoro, S., Tanaka, M., and Hirashima, N.
Cell. Immunol. 2009 258: 204-211.

2007

SCRAPPER-dependent ubiquitination of active zone protein RIM1 regulates synaptic vesicle release.

Yao, I., Takagi, H., Ageta, H., Kahyo, T., Sato, S., Hatanaka, K., Fukuda, Y., Chiba, T., Morone, N., Yuasa, S., Inokuchi, K., Ohtsuka, T., MacGregor, G.R., Tanaka, K., and Setou, M.
Cell 2007 130: 943-957.

Interaction between Munc13-1 and RIM is critical for glucagon-like peptide-1 mediated rescue　of exocytotic defects in Munc13-1 deficient pancreatic beta-cells.

Kwan, E.P., Xie, L., Sheu, L., Ohtsuka, T., and Gaisano, H.Y.
Diabetes 2007 56: 2579-2588.

Rab6　regulates transport and targeting of exocytotic carriers.

Grigoriev, I., Splinter, D., Keijzer, N., Wulf, P.S., Demmers, J., Ohtsuka, T., Modesti, M., Maly, I.V., Grosveld, F., Hoogenraad, C.C., and Akhmanova, A.
Dev. Cell 2007 13: 305-314.

Three-dimensional architecture of presynaptic terminal cytomatrix

Siksou, L., Rostaing, P., Lechaire, J.P., Boudier, T., Ohtsuka, T., Fejtov, A., Kao, H.T., Greengard, P., Gundelfinger, E.D., Triller, A., and Marty, S.
J. Neurosci. 2007 27: 6868-6877.

The active zone protein CAST directly associates with Ligand-of-Numb protein X.

Higa, S., Tokoro, T., Inoue, E., Kitajima, I., and Ohtsuka, T.
Biochem. Biophys. Res. Commun. 2007 354: 686-692.

Localization of the active zone proteins CAST, ELKS, and Piccolo at neuromuscular junctions.

Tokoro, T., Higa Onaga, S., Deguchi-Tawarada, M., Inoue, E., Kitajima, I., and Ohtsuka, T.
Neuroreport　2007 18: 313-316.

2006

CLASPs attach microtubele plus ends to the cell cortex through a complex with LL5beta.

Lansbergen, G., Grigoriev, I., Mimori-Kiyosue, Y., Ohtsuka, T., Higa, S., Kitajima, I., Demmers, J., Galjart, N., Houtsmuller, A.B., Grosveld, F., and Akhmanova, A.
Dev. Cell 2006 11: 21-32.

SAD: a presynaptic kinase associated with synaptic vesicles and the active zone cytomatrix that regulates neurotransmitter release.

Inoue, E., Mochida, S., Takagi, H., Higa, S., Deguchi-Tawarada, M., Takao-Rikitsu, E., Inoue, M., Yao, I., Takeuchi, K., Kitajima, I., Setou, M., Ohtsuka, T.*, and Takai, Y.
Neuron 2006 50: 261-275.(*corresponding author)

ELKS, a protein structurally related to the active zone protein CAST, is involved in Ca2+-dependent exocytosis from PC12 cells.

Inoue, E., Deguchi-Tawarada, M., Takao-Rikitsu, E., Inoue, M., Kitajima, I., Ohtsuka, T.*, and Takai, Y.
Genes Cells 2006 11: 659-672.

Active zone protein CAST is a component of conventional and ribbon synapses in mouse retina.

Deguchi-Tawarada, M., Inoue, E., Takao-Rikitsu, E., Inoue, M., Kitajima, I., Ohtsuka, T.*, and Takai, Y.
J. Comp. Neurol. 2006 495: 480-496.(*corresponding author)

2005

ELKS, a protein structurally related to the active zone-associated protein CAST, is expressed in pancreatic beta cells and functions in insulin exocytosis: interaction of ELKS with exocytotic machinery analyzed by total internal reflection fluorescence microscopy.

Ohara-Imaizumi, M., Ohtsuka, T., Matsushima, S., Akimoto, Y., Nishiwaki, C., Nakamichi, Y., Kikuta, T., Nagai, S., Kawakami, H., Watanabe, T., and Nagamatsu, S.
Mol. Biol. Cell 2005 16: 3289-3300.

Differential distribution of release-related proteins in the hippocampal CA3 area as revealed by freeze-fracture replica labeling.

Hagiwara, A., Fukazawa, Y., Deguchi-Tawarada, M., Ohtsuka, T., and Shigemoto, R.
J. Comp. Neurol. 2005 489: 195-216.

Nectin-like molecule-1/TSLL1/SynCAM3 : a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule localizing at non-junctional contact sites of presynaptic nerve terminals, axons and glia cell processes.

Kakunaga S., Ikeda W., Itoh, S., Deguchi-Tawarada, M., Ohtsuka, T., Mizoguchi, A., and Takai, Y.
J. Cell Sci. 2005 118: 1267-1277.

Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development.

Inagaki, M., Irie, K., Ishizaki, H., Tanaka-Okamoto, M., Morimoto, K., Inoue, E., Ohtsuka, T., Miyoshi, J., and Takai, Y.
Development 2005 132: 1525-1537.

2004

Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release.

Takao-Rikitsu, E., Mochida, S., Inoue, E., Deguchi-Tawarada, M., Inoue, M., Ohtsuka, T.*, and Takai, Y.
J. Cell Biol. 2004 164: 301-311.(*corresponding author)

CAST2: identification and characterization of a protein structurally related to the presynaptic cytomatrix protein CAST.

Deguchi-Tawarada, M., Inoue, E., Takao-Rikitsu, E., Inoue, M., Ohtsuka, T.*, and Takai, Y.
Genes Cells 2004 9: 15-23. (*corresponding author)

2003

Nectin-dependent localization of synaptic scaffolding molecule (S-SCAM) at the puncta adherentia junctions formed between the mossy fibre terminals and the dendrites of pyramidal cells in the CA3 area of the mouse hippocampus.

Yamada, A., Irie, K., Deguchi-Tawarada, M., Ohtsuka, T., and Takai, Y.
Genes Cells 2003 8: 985-994.

The roles of cadherins and nectins in interneuronal synapse formation.

Takai, Y., Shimizu, K., and Ohtsuka, T.
Curr. Opin. Neurobiol. 2003 5: 520-526. Review.

Nectin-dependent localization of ZO-1 at puncta adhaerentia junctions between the mossy fiber terminals and the dendrites of the pyramidal cells in the CA3 area of adult mouse hippocampus.

Inagaki, M., Irie, K., Deguchi-Tawarada, M., Ikeda, W., Ohtsuka, T., Takeuchi, M.,and Takai, Y.
J. Comp. Neurol. 2003 460: 514-524.

2002

CAST: a novel protein of the cytomatrix at the active zone of synapses that forms a ternary complex with RIM1 and Munc13-1.

Ohtsuka, T.*, Takao-Rikitsu, E., Inoue, E., Inoue, M., Takeuchi, M., Matsubara, K., Deguchi-Tawarada, M., Satoh, K., Morimoto, K., Nakanishi, H., and Takai, Y.
J. Cell Biol. 2002 158: 577-590.(*corresponding author)

2000

Membrane-associated guanylate kinase with inverted orientation (MAGI)-1/brain angiogenesis inhibitor 1-associated protein (BAP1) as a scaffolding molecule for Rap small G protein GDP/GTP exchange protein at tight junctions.

Mino, A., Ohtsuka, T., Inoue, E., and Takai, Y.
Genes Cells 2000 5: 1009-1016.

Association of membrane-associated guanylate kinase-interacting protein-1 with Raf-1.

Yao, I., Ohtsuka, T., Kawabe, H., Matsuura, Y., Takai, Y., and Hata, Y.
Biochem. Biophys. Res. Commun. 2000 270: 538-542.

Importance of spatial activation of Cdc42 and rac small G proteins by frabin for microspike formation in MDCK cells.

Yasuda, T., Ohtsuka, T., Inoue, E., Yokoyama, S., Sakisaka, T., Kodama, A., Takaishi, K., and Takai, Y.
Genes Cells 2000 5: 583-591.

1999

nRap GEP: a novel neural GDP/GTP exchange protein for rap1 small G protein that interacts with synaptic scaffolding molecule (S-SCAM).

Ohtsuka, T., Hata, Y., Ide, N., Yasuda, T., Inoue, E., Inoue, T., Mizoguchi, A., and Takai, Y.
Biochem. Biophys. Res. Commun. 1999 265: 38-44.

Rab11BP/Rabphilin-11, a downstream target of rab11 small G protein implicated in vesicle recycling.

Mammoto, A., Ohtsuka, T., Hotta, I., Sasaki, T., and Takai, Y.
J. Biol. Chem. 1999 274: 25517-25524.

1998

Nexilin: a novel actin filament-binding protein localized at cell-matrix adherens junction.

Ohtsuka, T., Nakanishi, H., Ikeda, W., Satoh, A., Momose, Y., Nishioka, H., and Takai, Y.
J. Cell Biol. 1998 143: 1227-1238.

Cell-free assay system for Ras- and Rap1-dependent activation of MAP-kinase cascade.

Shimizu, K., Ohtsuka, T., and Takai, Y.
Methods Mol. Biol. 1998 84: 173-183. Review.

1996

Different effects of various phospholipids on Ki-Ras-, Ha-Ras-, and Rap1B-induced B-Raf activation.

Kuroda, S., Ohtsuka, T., Yamamori, B., Fukui, K., Shimizu, K., and Takai, Y.
J. Biol. Chem. 271: 14680-14683.

Activation of brain B-Raf protein kinase by Rap1B small GTP-binding protein.

Ohtsuka, T., Shimizu, K., Yamamori, B., Kuroda, S., and Takai, Y.
J. Biol. Chem. 1996 271: 1258-1261.

1995

Purification of a Ras-dependent mitogen-activated protein kinase kinase kinase from bovine brain cytosol and its identification as a complex of B-Raf and 14-3-3 proteins.

Yamamori, B., Kuroda, S., Shimizu, K., Fukui, K., Ohtsuka, T., and Takai, Y.
J. Biol. Chem. 1995 270: 11723-11726.

Doc2: a novel brain protein having two repeated C2-like domains.

Orita, S., Sasaki, T., Naito, A., Komuro, R., Ohtsuka, T., Maeda, M., Suzuki, H., Igarashi, H., and Takai, Y.
Biochem. Biophys. Res. Commun. 1995 206: 439-448.