Publication

2019

  • UBAP1 mutations cause juvenile-onset hereditary spastic paraplegias (SPG80) and impair UBAP1 targeting to endosomes.
    Nan, H., Ichinose, Y., Tanaka, M., Koh, K., Ishiura, H., Mitsui, J., Mizukami, H., Morimoto, M., Hamada, S., Ohtsuka, T., Tsuji, S., Takiyama, Y.
    J Hum Genet. 2019. doi.10.1038/s10038-019-0670-9.



  • Role of the active zone protein, ELKS, in insulin secretion from pancreatic β-cells.
    Ohara-Imaizumi M, Aoyagi K, Ohtsuka, T.,
    Mol Metab. 2019. doi:10.1016/j.molmet.2019.06.017.



  • Vangl2 interaction plays a role in the proteasomal degradation of Prickle2.
    Nagaoka, T., Furuse, M., Ohtsuka, T.,Tsuchida, K., Kishi, M.
    Scientific Reports 9. 2019. doi.org/10.1038/s41598-019-39642-z



  • ELKS/Voltage-dependent Ca2+ channel-β Subunit Module Regulates Polarized Ca2+ Influx in Pancreatic β-Cells.
    Ohara-Imaizumi, M.*, Aoyagi, K., Hida, Y., Yoshida, M., Yamauchi, H., Ohkura, M., Abe, M., Akimoto, Y., Nakamichi, Y., Nishiwaki, C., Kawakami, H., Hara, K., Sakimura, K., Nakai, J., Kakei, M., Nagamatsu, S., Ohtsuka, T.*.
    Cell Reports 26: 1213-1226. 2019. doi.org/10.1016/j.celrep.2018.12.106



  • 2018

  • Cytomatrix proteins CAST and ELKS regulate retinal photoreceptor development and maintenance.
    Hagiwara, A., Kitahara, Y., Grabner, C.P., Vogl, C., Abe, M., Kitta, R., Ohta, K., Nakamura, K., Sakimura, K., Moser, T., Nishi, A., Ohtsuka, T.*.
    J. Cell Biol. 28(1), 3993-4006, 2018. doi: 10.1083/jcb.201704076.



  • CAST/ELKS proteins control voltage-gated Ca2+ channel density and synaptic release probability at a mammalian central synapse.
    Dong, W., Radulovic, T., Thomas, C., Montesinos, M.S., Goral, R.O., Given, D.G., Hagiwara, A., Putzke, T., Hida, Y., Abe, M., Sakimura, K., Kamasawa, N., Ohtsuka, T.*, Young, S.M*.
    Cell Reports. 24(2), 284-293.e6, 2018. doi: 10.1016/j.celrep.2018.06.024.



  • Neuroimaging, genetic, and enzymatic study in a Japanese family with a GBA gross deletion.
    Ichinose, Y., Ishiura, H., Tanaka, M., Yoshimura, J., Doi, K., Umeda, T., Yamauchi, H., Tsuchiya, M., Koh, K., Yamashiro, N., Mitsui, J., Goto, J., Onishi, H., Ohtsuka, T., Shindo, K., Morishita, S., Tsuji, S., Takiyama, Y.
    Parkinsonism & Related Disorders 2018. doi:org/10.1016/j.parkreldis.2018.11.028



  • CAST/ELKS proteins control voltage-gated Ca2+ channel density and synaptic release probability at a mammalian central synapse.
    Dong, W., Radulovic, T., Thomas, C., Montesinos, M.S., Goral, R.O., Given, D.G., Hagiwara, A., Putzke, T., Hida, Y., Abe, M., Sakimura, K., Kamasawa, N., Ohtsuka, T.*, Young, S.M*.
    Cell Reports. 24(2), 284-293.e6, 2018. doi: 10.1016/j.celrep.2018.06.024.



  • Mutations in bassoon in individuals with familial and sporadic progressive supranuclear palsy-like syndrome.
    Yabe, I., Yaguchi, H., Kato, Y., Miki, Y., Takahashi, H., Tanikawa, S., Shirai, S., Takahashi, I., Kimura, M., Hama, Y., Matsushima, M., Fujioka, S., Kano, T., Watanabe, M., Nakagawa, S., Kunieda, Y., Ikeda, Y., Hasegawa, M., Nishihara, H., Ohtsuka, T., Tanaka, S., Tsuboi, Y., Hatakeyama, S., Wakabayashi, K., Sasaki, H.
    Scientific Reports. 8(1), 819, 2018. doi: 10.1038/s41598-018-19198-0.



  • Synaptic localisation of SRF coactivators, MKL1 and MKL2, and their role in dendritic spine morphology.
    Kaneda, M., Sakagami, H., Hida, Y., Ohtsuka, T., Satou, N., Ishibashi, Y., Fukuchi, M., Krysiak, A., Ishikawa, M., Ihara, D., Kalita, K., Tabuchi, A.
    Scientific Reports. 8(1), 727, 2018. doi: 10.1038/s41598-017-18905-7.



  • CAST: its molecular structure and phosphorylation-dependent regulation of presynaptic plasticity.
    Hamada, S., Ohtsuka, T.
    Neurosci. Res. 127, 25-32, 2018. doi: 10.1016/j.neures.2017.12.005.



  • 2017

  • Opposing expression of Wnt5a/5b and their inhibitors Sfrps followed by intercellular signaling via PCP proteins polarize node cells for left-right symmetry breaking.
    Minegishi, K., Hashimoto, M., Ajima, R., Takaoka, K., Shinohara, K., Ikawa, Y., Nishimura, H., McMahon, A., Willert, K., Sasaki, H., Fujimori, T.,Ohtsuka, T., Yamaguchi, T., Shimono, A., Shiratori, H., and Hamada, H.
    Developmental Cell 40(5), 439-452, 2017.

  • Auxiliary calcium channel subunit α2δ4 is required for axonal elaboration, synaptic transmission, and wiring of rod photoreceptors.
    Wang, Y., Fehlhaber, K.E., Sarria, I., Cao, Y., Ingram, N.T., Guerrero-Given, D., Throesch, B., Baldwin, K., Kamasawa, N.,Ohtsuka, T., Sampath, A.P., Martemyanov, K.A.
    Neuron 93(6),1359-1374, 2017.

  • 2016

    • A de novo mutation in the NALCN gene in an adult patient with cerebellar ataxia associated with intellectual disability and arthrogryposis.
      Wang, Y. Koh, K., Ichinose, Y., Yasumura, M., Ohtsuka, T., Takiyama Y.
      CLINICAL GENETICS 90(6): 556-557, 2016.

    • SAD-B Phosphorylation of CAST Controls Active Zone Vesicle Recycling for Synaptic Depression.
      Mochida, S.*, Hida, Y., Tanifuji, S., Hagiwara, A., Hamada, S., Abe, M., Huan, M., Yasumura, M., Kitajima, I., Sakimura, K.,Ohtsuka T.*.(*corresponding authors)
      Cell Reports 16(11): 2901-2913, 2016.

    • The active zone protein CAST regulates synaptic vesicle recycling and quantal size in the mouse hippocampus.
      Kobayashi, S., Hida, Y., Ishizaki, H., Inoue, E.,Tanaka-Okamoto, M., Yamasaki, M., Miyazaki, T.,Fukaya, M., Kitajima, I., Takai, Y., Watanabe, M., Ohtsuka, T.*, Manabe, T.*(*corresponding authors)
      European Journal of Neuroscience 44(5): 2272-2284,2016.

    • Dopamine synapse is a neuroligin-2–mediated contact between dopaminergic presynaptic and GABAergic postsynaptic structures.
      Uchigashima, M., Ohtsuka T., Kobayashi, K., Watanabe, M.
      PNAS 2016 113(15): 4206-4211. doi: 10.1073/pnas.1514074113

    2015

    • SAD-B kinase regulates presynaptic vesicular dynamics at hippocampal Schaffer collateral synapses and affects contextual fear memory.
      Watabe, A.M., Nagase, M., Hagiwara, A., Hida, Y., Tsuji, M., Ochiai, T., Kato, F., Ohtsuka, T.
      J. Neurochem. 2016 136(1): 36-47. doi: 10.1111/jnc.13379.

    2014

    • The planar cell polarity protein Vangl2 bidirectionally regulates dendritic branching in cultured hippocampal neurons.
      Hagiwara A, Yasumura M, Hida Y, Inoue E, Ohtsuka T.
      Mol. Brain 2014 Nov 12;7(1):79

    2013

    • Identification, expression and characterization of rat isoforms of the serum response factor (SRF) coactivator MKL1.
      Ishikawa M., Shiota J., Ishibashi Y., Hakamata T., Shoji S., Fukuchi M., Tsuda M., Shirao T., Sekino Y., Ohtsuka T., Baraban JM., Tabuchi A.
      FEBS Open Bio. 2013 387-393.

    • Vangl2, the planar cell polarity protein, is complexed with postsynaptic density protein PSD-95
      Yoshioka T., Hagiwara A, Hida Y, Ohtsuka T.
      FEBS letters 2013 587:1453-1459.

    • CAST: functional scaffold for the integrity of the presynaptic active zone
      Ohtsuka T.
      Neurosci. Res 2013 76:10-15

    2012

    • Deletion of the Presynaptic Scaffold CAST Reduces Active Zone Size in RodPhotoreceptors and Impairs Visual Processing
      tom Dieck S., Specht D., Strenzke N., Hida Y., Krishnamoorthy V., SchmidtK.F., Inoue E., Ishizaki H., Tanaka-Okamoto M., Miyoshi J., Hagiwara A.,Brandstatter J.H., Lowel S, Gollisch T., Ohtsuka T.* and Moser T.*
      J. Neurosci 2012 32: 12192-12203.(*corresponding author)
    • Physical and Functional Interaction of the Active Zone Protein CAST/ERC2and the β-subunit of the Voltage-dependent Ca 2+ Channel.
      Kiyonaka S, Nakajima H, Takada Y, Hida Y, Yoshioka T, Hagiwara A, KitajimaI, Mori Y and Ohtsuka T.
      J. Biochem 2012 159:149-159.

    2011

    • Distribution of serine/threonine kinase SAD-B in mouse peripheral nervesynapse.
      Hagiwara A, Harada K, Hida Y, Kitajima I, Ohtsuka, T.
      Neuroreport 2011 22: 319-325.
    • Prickle2 is localized in the postsynaptic density and interacts with PSD-95and NMDA receptors in the brain.
      Hida Y, Fukaya M, Hagiwara A, Deguchi-Tawarada M, Yoshioka T, KitajimaI, Inoue E, Watanabe M, and Ohtsuka T
      J. Biochem. 2011 149: 693-700.

    2010

    • CAST and ELKS proteins: structural and functional determinants of the presynapticactive zone.
      Hida, Y.,Ohtsuka, T.
      J. Biochem. 2010 148: 131-137.
    • Reduced expression of the ATRX gene, a chromatin-remodeling factor, causeshippocampal dysfunction in mice.
      Nogami, T., Beppu, H., Tokoro, T., Moriguchi, S., Shioda, N., Fukuaga,K., Ohtsuka, T., Ishii, I., Sasahara, M., Shimada, Y., Nishijo, H., Li, E., and
      Kitajima, I.
      Hippocampus 2010 Mar 15. [Epub ahead of print]

    2009

    • Involvement of ELKS, an active zone protein, in exocytotic release fromRBL-2H3 cells.
      Nomura, H., Ohtsuka, T., Tadokoro, S., Tanaka, M., and Hirashima, N.
      Cell. Immunol. 2009 258: 204-211.

    2007

    • SCRAPPER-dependent ubiquitination of active zone protein RIM1 regulatessynaptic vesicle release.
      Yao, I., Takagi, H., Ageta, H., Kahyo, T., Sato, S., Hatanaka, K., Fukuda,Y., Chiba, T., Morone, N., Yuasa, S., Inokuchi, K., Ohtsuka, T., MacGregor, G.R., Tanaka, K., and Setou, M.
      Cell
      2007 130: 943-957.
    • Interaction between Munc13-1 and RIM is critical for glucagon-like peptide-1mediated rescue of exocytotic defects in Munc13-1 deficient pancreaticbeta-cells.
      Kwan, E.P., Xie, L., Sheu, L., Ohtsuka, T., and Gaisano, H.Y.
      Diabetes 2007 56: 2579-2588.
    • Rab6 regulates transport and targeting of exocytotic carriers. Grigoriev,I., Splinter, D., Keijzer, N., Wulf, P.S., Demmers, J., Ohtsuka, T., Modesti, M., Maly, I.V., Grosveld, F., Hoogenraad, C.C., and Akhmanova,A.
      Dev. Cell 2007 13: 305-314.
    • Three-dimensional architecture of presynaptic terminal cytomatrix.
      Siksou, L., Rostaing, P., Lechaire, J.P., Boudier, T., Ohtsuka, T., Fejtov, A., Kao, H.T., Greengard, P., Gundelfinger, E.D., Triller, A.,and Marty, S.
      J. Neurosci. 2007 27: 6868-6877.
    • The active zone protein CAST directly associates with Ligand-of-Numb proteinX.
      Higa, S., Tokoro, T., Inoue, E., Kitajima, I., and Ohtsuka, T.
      Biochem. Biophys. Res. Commun. 2007 354: 686-692.
    • Localization of the active zone proteins CAST, ELKS, and Piccolo at neuromuscularjunctions.
      Tokoro, T., Higa Onaga, S., Deguchi-Tawarada, M., Inoue, E., Kitajima,I., and Ohtsuka, T.
      Neuroreport 2007 18: 313-316.

    2006

    • CLASPs attach microtubele plus ends to the cell cortex through a complex with LL5beta.
      Lansbergen, G., Grigoriev, I., Mimori-Kiyosue, Y., Ohtsuka, T., Higa, S., Kitajima, I., Demmers, J., Galjart, N., Houtsmuller, A.B., Grosveld, F., and Akhmanova, A.
      Dev. Cell 2006 11: 21-32.
    • SAD: a presynaptic kinase associated with synaptic vesicles and the active zone cytomatrix that regulates neurotransmitter release.
      Inoue, E., Mochida, S., Takagi, H., Higa, S., Deguchi-Tawarada, M., Takao-Rikitsu, E., Inoue, M., Yao, I., Takeuchi, K., Kitajima, I., Setou, M., Ohtsuka, T.*, and Takai, Y.
      Neuron2006 50: 261-275.(*corresponding author)
    • ELKS, a protein structurally related to the active zone protein CAST, is involved in Ca2+-dependent exocytosis from PC12 cells.
      Inoue, E., Deguchi-Tawarada, M., Takao-Rikitsu, E., Inoue, M., Kitajima, I., Ohtsuka, T.*, and Takai, Y.
      Genes Cells 2006 11: 659-672.
    • Active zone protein CAST is a component of conventional and ribbon synapses in mouse retina.
      Deguchi-Tawarada, M., Inoue, E., Takao-Rikitsu, E., Inoue, M., Kitajima, I., Ohtsuka, T.*, and Takai, Y.
      J. Comp. Neurol.2006 495: 480-496.(*corresponding author)

    2005

    • ELKS, a protein structurally related to the active zone-associated protein CAST, is expressed in pancreatic beta cells and functions in insulin exocytosis: interaction of ELKS with exocytotic machinery analyzed by total internal reflection fluorescence microscopy.
      Ohara-Imaizumi, M., Ohtsuka, T., Matsushima, S., Akimoto, Y., Nishiwaki, C., Nakamichi, Y., Kikuta, T., Nagai, S., Kawakami, H., Watanabe, T., and Nagamatsu, S.
      Mol. Biol. Cell 2005 16: 3289-3300.
    • Differential distribution of release-related proteins in the hippocampal CA3 area as revealed by freeze-fracture replica labeling.
      Hagiwara, A., Fukazawa, Y., Deguchi-Tawarada, M., Ohtsuka, T., and Shigemoto, R.
      J. Comp. Neurol. 2005 489: 195-216.
    • Nectin-like molecule-1/TSLL1/SynCAM3 : a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule localizing at non-junctional contact sites of presynaptic nerve terminals, axons and glia cell processes.
      Kakunaga S., Ikeda W., Itoh, S., Deguchi-Tawarada, M., Ohtsuka, T., Mizoguchi, A., and Takai, Y.
      J. Cell Sci. 2005 118: 1267-1277.
    • Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development.
      Inagaki, M., Irie, K., Ishizaki, H., Tanaka-Okamoto, M., Morimoto, K., Inoue, E., Ohtsuka, T., Miyoshi, J., and Takai, Y.
      Development 2005 132: 1525-1537.

    2004

    • Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release.
      Takao-Rikitsu, E., Mochida, S., Inoue, E., Deguchi-Tawarada, M., Inoue, M., Ohtsuka, T.*, and Takai, Y.
      J. Cell Biol. 2004 164: 301-311.(*corresponding author)
    • CAST2: identification and characterization of a protein structurally related to the presynaptic cytomatrix protein CAST.
      Deguchi-Tawarada, M., Inoue, E., Takao-Rikitsu, E., Inoue, M., Ohtsuka, T.*, and Takai, Y.
      Genes Cells 2004 9: 15-23. (*corresponding author)

    2003

    • Nectin-dependent localization of synaptic scaffolding molecule (S-SCAM) at the puncta adherentia junctions formed between the mossy fibre terminals and the dendrites of pyramidal cells in the CA3 area of the mouse hippocampus.
      Yamada, A., Irie, K., Deguchi-Tawarada, M., Ohtsuka, T., and Takai, Y.
      Genes Cells 2003 8: 985-994.
    • The roles of cadherins and nectins in interneuronal synapse formation.
      Takai, Y., Shimizu, K., and Ohtsuka, T.
      Curr. Opin. Neurobiol. 2003 5: 520-526. Review.
    • Nectin-dependent localization of ZO-1 at puncta adhaerentia junctions between the mossy fiber terminals and the dendrites of the pyramidal cells in the CA3 area of adult mouse hippocampus.
      Inagaki, M., Irie, K., Deguchi-Tawarada, M., Ikeda, W., Ohtsuka, T., Takeuchi, M.,and Takai, Y.
      J. Comp. Neurol. 2003 460: 514-524.

    2002

    • CAST: a novel protein of the cytomatrix at the active zone of synapses that forms a ternary complex with RIM1 and Munc13-1.
      Ohtsuka, T.*, Takao-Rikitsu, E., Inoue, E., Inoue, M., Takeuchi, M., Matsubara, K., Deguchi-Tawarada, M., Satoh, K., Morimoto, K., Nakanishi, H., and Takai, Y.
      J. Cell Biol. 2002 158: 577-590.(*corresponding author)

    2000

    • Membrane-associated guanylate kinase with inverted orientation (MAGI)-1/brain angiogenesis inhibitor 1-associated protein (BAP1) as a scaffolding molecule for Rap small G protein GDP/GTP exchange protein at tight junctions.
      Mino, A., Ohtsuka, T., Inoue, E., and Takai, Y.
      Genes Cells 2000 5: 1009-1016.
    • Association of membrane-associated guanylate kinase-interacting protein-1 with Raf-1.
      Yao, I., Ohtsuka, T., Kawabe, H., Matsuura, Y., Takai, Y., and Hata, Y.
      Biochem. Biophys. Res. Commun. 2000 270: 538-542.
    • Importance of spatial activation of Cdc42 and rac small G proteins by frabin for microspike formation in MDCK cells.
      Yasuda, T., Ohtsuka, T., Inoue, E., Yokoyama, S., Sakisaka, T., Kodama, A., Takaishi, K., and Takai, Y.
      Genes Cells 2000 5: 583-591.

    1999

    • nRap GEP: a novel neural GDP/GTP exchange protein for rap1 small G protein that interacts with synaptic scaffolding molecule (S-SCAM).
      Ohtsuka, T., Hata, Y., Ide, N., Yasuda, T., Inoue, E., Inoue, T., Mizoguchi, A., and Takai, Y.
      Biochem. Biophys. Res. Commun. 1999 265: 38-44.

    1998

    • Nexilin: a novel actin filament-binding protein localized at cell-matrix adherens junction.
      Ohtsuka, T., Nakanishi, H., Ikeda, W., Satoh, A., Momose, Y., Nishioka, H., and Takai, Y.
      J. Cell Biol. 1998 143: 1227-1238.
    • Cell-free assay system for Ras- and Rap1-dependent activation of MAP-kinase cascade.
      Shimizu, K., Ohtsuka, T., and Takai, Y.
      Methods Mol. Biol. 1998 84: 173-183. Review.

    1996

    • Different effects of various phospholipids on Ki-Ras-, Ha-Ras-, and Rap1B-induced B-Raf activation.
      Kuroda, S., Ohtsuka, T., Yamamori, B., Fukui, K., Shimizu, K., and Takai, Y.
      J. Biol. Chem. 271: 14680-14683.
    • Activation of brain B-Raf protein kinase by Rap1B small GTP-binding protein.
      Ohtsuka, T., Shimizu, K., Yamamori, B., Kuroda, S., and Takai, Y.
      J. Biol. Chem. 1996 271: 1258-1261.

    1995

    • Purification of a Ras-dependent mitogen-activated protein kinase kinase kinase from bovine brain cytosol and its identification as a complex of B-Raf and 14-3-3 proteins.
      Yamamori, B., Kuroda, S., Shimizu, K., Fukui, K., Ohtsuka, T., and Takai, Y.
      J. Biol. Chem. 1995 270: 11723-11726.
    • Doc2: a novel brain protein having two repeated C2-like domains.
      Orita, S., Sasaki, T., Naito, A., Komuro, R., Ohtsuka, T., Maeda, M., Suzuki, H., Igarashi, H., and Takai, Y.
      Biochem. Biophys. Res. Commun. 1995 206: 439-448.